Caffeine by Polarized Light Microscopy

About:

It would be difficult not to be aware of Caffeine, which is a central nervous system stimulant. Interestingly it is apparently the worlds most widely consumed psychoactive drug but remains legal and unregulated in nearly all parts of the world.

Caffeine functions in several ways, the most commonly known is where it reversibly blocks the action of adenosine on a receptor which prevents the onset of drowsiness as a result of adenosine.  Beyond that it is also reported to stimulate portions of the autonomic nervous system.

Caffeine itself is a white crystalline purine sound in the seeds, nuts and leaves of a number of plants which are native to South America and East Asia. Once extracted it is used as an additive in a number of products including Tea, Coffee and Cola.

How it was done:

Since caffeine has a relatively low melting point (

The image was then captured using my normal polarized microscopy setup, which included;

Because the depth of field is relatively small, some images did require focus stacking which is where Helicon Remote (affiliate-link) & Focus came into the picture (pardon the pun).

Images: (Click to enlarge)

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TravisH @travishale

Passionate scientist, and hobby photographer making my way through the world with the help of Family and Friends.

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