This episode of under the microscope focuses on “Onion” cells, the cellular level of a commonly used kitchen ingredient in a whole range of cuisines. The onion (Allium Cepa) is a vegetable that has been selectively grown and bread for more than 7,000 years; It is biennial but typically is grown as an annual with yellowish-green leaves. As the onion matures, food reserves begin to accumulate in the leaf bases and the bulb of the onion (the part we eat) begins to swell.
How it was done:
All of my “under the microscope” images are taken in a similar manner, and using the below equipment. In this case, focus stacking was conducted by capturing a video of the subject as I move through the planes of focus, and then software pulled the images out of that video and focus stacked them into the images below;
Images captured are displayed below, Sadly the top right is slightly out of focus but you can see the other details are more focussed.
Image 1: Onion cells at around 4x magnification, focus stacked using Helicon Focus.
Image 2: Onion cells at around 10x magnification, focus stacked using Helicon Focus.
Image 3: Onion cells at around 40x magnification, focus stacked using Helicon Focus.